Composite

Part:BBa_K3467013

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-25)


T7+CHS dsRNA sequence

CHS is gene of chitin synthase ,with 5116bp cDNA sequence, which has a critical function on locust ecdysis. We designed the dsRNA sequence of CHS by using specific program(https://www.flyrnai.org/cgi-bin/RNAi_find_primers.pl) and find a 669bp length of CHS dsRNA for RNAi.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 611
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 611
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 671
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 611
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 611
  • 1000
    COMPATIBLE WITH RFC[1000]


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